Wednesday, 21 August 2013

It is all in the name…. Why am I (not) Dr Nick?

In Malaysia, I am called Dr Nick.... And I couldn't understand why... Today, I found out.

In Malaysia, there are no family names, as in Morris, Smith, Jones etc. Your last name is your father's first name, so if my father's first name was Ian, then I would be Nick Ian. Hence, as I am Dr Nick Morris, this suggests that my father's first name was Morris, and Dr Morris would be my father!

This can get odd for a woman, as they would be Julie Ian, and so would Ms Ian if the European convention was followed.

Thanks to The Simpsons, Dr. Nick has a certain 'feeling' about it. You can read more about Dr. Nick of Simpson's fame on Wikipedia.

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Thursday, 28 February 2013

Paper published - Adiponectin corrects high fat diet-induced disturbances in muscle metabolomic profile and whole body glucose homeostasis

Great to finally see a paper that I was working on last year out in print….

Liu,Y., Turdi,S., Park,T., Morris,N.J., Deshaies,Y., Xu,A. & Sweeney,G. Adiponectin corrects high fat diet-induced disturbances in muscle metabolomic profile and whole body glucose homeostasis (2013) Diabetes 62 743-752

Pubmed Link: http://www.ncbi.nlm.nih.gov/pubmed/23238294

There is also a very nice commentary (subscription required) at: http://diabetes.diabetesjournals.org/content/62/3/701.full

The others did all the hard work; I just applied my geeky computing skills to crunch and analyse all the lovely data that was produced. Happy days!

Wednesday, 14 November 2012

Glycogen Synthase Kinase 3 (GSK3) and Glycogen Synthase (GS) - the phosphorylation equilibrium

This one causes students problems every year - the relationship between Glycogen Synthase Kinase 3 (GSK3) and Glycogen Synthase (GS), and their phosphorylation states and activity.

If you look at insulin signalling overall:

Is

You will see that activated protein kinase B (activated by phosphorylation) phosphorylates and inactivates GSK3. This means that GSK3 can no longer phosphorylate its substrate, GS.

Now, GS, when phosphorylated, is inactive. Hence, as GSK3 is no longer active (as it is phosphorylated) it cannot phosphorylate GS and inactivate it, therefore GS remains un-phosphorylated and therefore active so it can make glycogen.

Gsk3

The key to understanding this is that phosphorylated (inactive) and un-phosphorylated (active) forms of GS are in a state of equilibrium and that the kinase, GSK3, drives the equilibrium to the right (inactive) in the figure below, whereas the phosphatase (which removes the phosphate) drives the equilibrium to the left (active). Therefore, if you inhibit the action of the kinase (GSK3) the net result is more un-phosphorylated GS, hence more glycogen is made, which is just what we want…

Phosphate copy

One key thing to keep in mind is that the phosphorylation of a protein can cause it to become active or inactive. It all depends on the protein.

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Saturday, 20 October 2012

Calculating Percentage Solutions

For some reason, percentage solution calculations cause students some problems. However, hopefully, once you have read this blog post, you should have a good understanding of percentage solutions and how to do the calculations.

If you want to practice your 'science maths', then have a look at Maths4Biosciences

What are percentage solutions?

A percentage solution is an amount or volume of something per 100 ml or 100 g of a solution. It is as simple as that. It is a percentage.

Why are they used?

Percentage solutions are a convenient and easy way to record solution concentrations. One advantage is that you don’t need to know anything about the compound in terms of molecular weight; all you need is the percentage of the required solution.

Why are there three types of percentage solutions?

This is slightly difficult to explain. However, there are three types of percentage solutions:
  • Percentage weight by volume (w/v)
  • Percentage volume by volume (v/v)
  • Percentage weight by weight (w/w)
The percentage weight by volume (w/v) is the number of grams of compound per 100 ml of solution. This type of percentage solution is used when describing the amount of powder in a solution. For example, 5 g of powder made up to a final volume of 100 ml would be a 5% (w/v) solution. Likewise, 2.5 g of powder made up to 50 ml would also be a 5% (w/v) solution, as you would have 5 g in 100 ml.

The percentage volume by volume (v/v) is the number of ml of some liquid per 100 ml of the solution. This type of percentage solution is usually used to describe a solution made by mixing two liquids. For example, 5 ml of a liquid made up to a final volume of 100 ml would be a 5% (v/v) solution. Likewise, 2.5 ml of a liquid made up to 50 ml would also be a 5% (v/v) solution, as you would have 5 ml in 100 ml.

Finally, the percentage weight by weight (w/w). This one is more difficult to understand, but the principles, as explained above, are still valid. A percentage weight by weight (w/w) solution can be the weight of a powder or a liquid made up in a solution to the final weight of the solution. So, for example, 5 g of a powder (or a liquid) made up in a solution with a final weight of 100 g would be a 5% (w/w) solution. Likewise, 2.5 g of a liquid or powder made up to give a solution that weighed 50 g would also be a 5% (w/w) solution, as you would have 5 g of the powder or liquid in 100 g of a solution.


 

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Tuesday, 16 October 2012

3d Structure of the GDP G-protein alpha subunit

The movie below shows the 3d structure of a guanosine diphosphate (GDP) bound G protein alpha-subunit.

Gdp alpha

The small green molecule in the middle is the bound GDP.

https://www.rcsb.org/structure/1TAG

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Bovine Rhodopsin

The structure of a G-protein coupled receptor is often shown as:

Screen Shot 2012 10 16 at 20 58 21

However, the crystal structure of bovine rhodopsin shows that the seven transmembrane-spanning domains actually form a tight core

Bovine rhodopsin

J. Mol. Biol. (2004) 342, 571–583 https://www.rcsb.org/structure/1U19

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3d Structure - β2AR-T4L

The structure of a G-protein coupled receptor is often shown as:

Screen Shot 2012 10 16 at 20 58 21

Which shows a receptor with the N-terminal outside the cell, seven transmembrane-spanning domains, 3 exoloops, 4 cytoloops and a C terminal inside the cell.

However, it is actually more compact, with the seven transmembrane spanning domains forming a tight core.

Beta2AR

Lower part = T4-lysozyme in place of cyto 3 Small green molecules = cholesterol; can just make out bound ligand in pocket
Science. 2007 318(5854): 1258–1265. https://www.rcsb.org/structure/2RH1

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