This video is in response to a question I have received on YouTube:

*“Thanks it is very helpful but can you present how to calculate slope and intercept in this curve”*

The question often gets asked about two of my other videos:

- How to calculate the size of a DNA band on a gel?
- How to Determine Protein Size on an SDS-PAGE Gel | Step-by-Step Tutorial

In the How to Calculate the Gradient (m) and Intercept (c) in y = mx + c | Gel Analysis Tutorial, I explain how to calculate the gradient (m) and intercept (c) in the linear equation y = mx + c. I explain three methods that can be used:

- Graph-based calculation
- Solving simultaneous equations
- Using Excel or Apple Numbers.

If you would like to say thanks for the video, then please feel free to buy me a coffee at https://www.buymeacoffee.com/drnickm

**Blog Bonus:**Free information sheet summarising the video and defining the key terms - download.

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