retention factorThis video - How to Determine Protein Size on an SDS-PAGE Gel | Step-by-Step Tutorial - is in response to questions I have had about a video in which I show how to calculate the size in base pairs of bands on a DNA gel.
In the video, I explain how to determine the molecular weight (kilodaltons; kDa) of a protein on an SDS-PAGE gel. The method involves constructing a table with the known molecular weights of protein markers, calculating their logarithmic values, measuring the distance each band travels and determining their retention factor (Rf) values.
I then show how to plot the graph and determine the size in kilodaltons (kDa) for the protein under investigation.
If you would like to say thanks for the video, then please feel free to buy me a coffee at https://www.buymeacoffee.com/drnickm
Additional Reading
The video was produced with help from the following resources:
- 📗 - The Biosciences Glossary
- 📗 - Molecular Biology of the Cell (Alberts) - (affiliate link)
- 📗 - Molecular Cell Biology (Lodish) - (affiliate link)
- 📗 - Biochemistry (Stryer) - (affiliate link)
- 📗 - Maths and Chemistry Refresher for Life and Biomedical Scientists
- 📗 - Catchup Chemistry (affiliate link to the book)
- 📗 - Catchup Maths and Stats (affiliate link to the book)
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