In this video, I examine the step-by-step process of cloning DNA into plasmids and address common challenges faced in the lab. I start by preparing the DNA and then move on to using restriction enzymes like EcoRI and HindIII. I explain the importance of choosing the correct enzyme pairs to prevent self-ligation and ensure the correct orientation of the insert. I also cover the blue-white selection method to verify successful cloning and discuss using different vectors for larger DNA segments.
If you would like to support my blogging efforts, then please feel free to buy me a coffee at https://www.buymeacoffee.com/drnickm
Blog Bonus: Free information sheet summarising the video and defining the key terms - download.
Additional Reading
The video was produced with help from the following resources:
- 📗 - The Biosciences Glossary
- 📗 - Molecular Biology of the Cell (Alberts) - (affiliate link)
- 📗 - Molecular Cell Biology (Lodish) - (affiliate link)
- 📗 - Biochemistry (Stryer) - (affiliate link)
