Wednesday 1 May 2024

New video posted: DNA Cloning - how to overcome some common problems

In this video, I examine the step-by-step process of cloning DNA into plasmids and address common challenges faced in the lab. I start by preparing the DNA and then move on to using restriction enzymes like EcoRI and HindIII. I explain the importance of choosing the correct enzyme pairs to prevent self-ligation and ensure the correct orientation of the insert. I also cover the blue-white selection method to verify successful cloning and discuss using different vectors for larger DNA segments. 


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Blog Bonus: Free information sheet summarising the video and defining the key terms - download.

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