I have just posted a video on how to determine the size of a protein on an SDS PAGE gel - How to Determine Protein Size on an SDS-PAGE Gel | Step-by-Step Tutorial.
Blog Bonus: Free step-by-step guide explaining the calculation - download.
The video is a response to two questions I was asked:
- How do I calculate the size of a protein on an SDS-PAGE gel?
- Can the DNA method be used for proteins?
In the video and the free step-by-step guide explaining the calculation, I go over the necessary steps for working out the size of a protein band on a gel, explain the Retention Factor (Rf value) and how to use it, and outline the method's limitations.
Feel free to leave any questions or comments below. If you need further clarification on any step or have suggestions for future topics, let me know!
If you would like to say thanks for the video, then please feel free to buy me a coffee at https://www.buymeacoffee.com/drnickm
Blog Bonus: Free step-by-step guide explaining the calculation - download.
Additional Reading
The video was produced with help from the following resources:
- 📗 - The Biosciences Glossary
- 📗 - Molecular Biology of the Cell (Alberts) - (affiliate link)
- 📗 - Molecular Cell Biology (Lodish) - (affiliate link)
- 📗 - Biochemistry (Stryer) - (affiliate link)
- 📗 - Maths and Chemistry Refresher for Life and Biomedical Scientists
- 📗 - Catchup Chemistry (affiliate link to the book)
- 📗 - Catchup Maths and Stats (affiliate link to the book)
